Overview

  • Product name
  • Description
    Rabbit polyclonal to IL8
  • Specificity
    The antibody reacts with human natural and recombinant IL-8 as assessed by ELISA. The antibody inhibits the biological activity of human native and recombinant IL-8.
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Fr, IP, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Recombinant full length protein (human)

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab7747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 17208399
WB 1/10. Detects a band of approximately 6-8 kDa (predicted molecular weight: 11.1 kDa). IL-8 appears to exist as a dimer in solution.
IHC-Fr Use at an assay dependent concentration. PubMed: 25120588
IP 1/10.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 26270987

Target

  • Function
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 9E3 antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG1 antibody
    see all

Images

  • ab7747 staining IL8 in rat gingival tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with cold 4% paraformaldehyde. To assess the impact of P. gingivalis GroEL on the inflammatory response in gingival tissue, the expression of IL8 was assessed. As shown, there was little IL8 expression in the area of the periodontal membrane (periodontal ligament) in the control and GST treatment groups, as indicated by the black arrows. In contrast, the injection of GroEL induced significant expression of IL8 in the periodontal membrane area of the rat gingiva.

    Antibody stains were developed via DAB/hydrogen peroxide reaction. Sections were subsequently counterstained with hematoxylin, dehydrated, and mounted. Finally, the slides were observed using light microscopy.

     

  • ab7747 staining IL8 in SVGA astrocytes transfected with a plasmid encoding Vpr by ICC/IF (Immunocytochemistry/immunofluorescence). The cells were stained for nucleus (blue), IL8 (green) and GFAP (red). Cells were fixed with ice-cold methanol/acetone (1:1) for 20 minutes at 20°C, washed 3x with PBS and permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at RT. Samples were incubated with ab7747 overnight in a humidified chamber. The cells were washed 5x with 0.1% Triton X-100 in PBS and incubated for 1h in secondary antibodies conjugated with Alexa Fluor 488 (Anti-Rabbit, 1:1000) at room temperature in the dark. All the antibodies were diluted in PBS containing 1% BSA.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 11.1 kDa


    Exposure time : 1 minute

References

This product has been referenced in:
  • Freitas LS  et al. In situ detection of Chlamydia pneumoniae, C. trachomatis, and cytokines among cardiovascular diseased patients from the Amazon region of Brazil. Infect Drug Resist 10:109-114 (2017). IHC-P ; Human . Read more (PubMed: 28435302) »
  • Li Y  et al. Effect of targeted silencing of IL-8 on in vitro migration and invasion of SKOV3 ovarian cancer cells. Oncol Lett 13:567-572 (2017). Read more (PubMed: 28356930) »

See all 22 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (Macaque Retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Macaque Retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 01 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Muller Cell Line MIO-M1)
Permeabilization
Yes - 0.1% TritonX-100
Specification
Muller Cell Line MIO-M1
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 01 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (HNSCC Tissue)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
HNSCC Tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Dr. Biswanath Majumder

Verified customer

Submitted Jul 01 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up