Anti-IL8 antibody [B-K8] (Phycoerythrin) (ab27369)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-IL8 antibody [B-K8] (Phycoerythrin)
See all IL8 primary antibodies -
DescriptionMouse monoclonal [B-K8] to IL8 (Phycoerythrin)
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Host speciesMouse
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ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
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SpecificityRecognises both natural and recombinant IL8.
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Tested applicationsSuitable for: Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant full length IL8 (Human)
Properties
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FormLyophilised:Reconstitute with 1 ml deionized water
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 5% BSA, PBS -
Concentration information loading... -
PurityIon Exchange Chromatography
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ClonalityMonoclonal
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Clone numberB-K8
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Myelomax63-Ag8.653
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab27369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent dilution. Use 10µl for 5 x 105 cells. (intra-cytoplasmic) |
Target
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FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
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Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
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Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
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Alternative names
- (Ala-IL-8)77 antibody
- (Ser-IL-8)72 antibody
- 9E3 antibody
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Protocols
References
ab27369 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"