Anti-IL9R antibody (ab123404)

Overview

  • Product name
  • Description
    Rabbit polyclonal to IL9R
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 169-199 of Human IL9R (NP_002177.2).

  • Positive control
    • Mouse liver tissue lysate, A549 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 57 kDa.
IHC-Fr 1/300.

Target

  • Function
    This is a receptor for interleukin-9.
  • Sequence similarities
    Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.
  • Domain
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization
    Cell membrane. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD129 antibody
    • IL-9 receptor antibody
    • IL-9R antibody
    • Il9r antibody
    • IL9R_HUMAN antibody
    • IL9RX antibody
    • IL9RY antibody
    • Interleukin 9 receptor antibody
    • Interleukin-9 receptor antibody
    see all

Images

  • Anti-IL9R antibody (ab123404) at 1/100 dilution + A549 cell lysate at 35 µg

    Predicted band size : 57 kDa
  • Anti-IL9R antibody (ab123404) at 1/100 dilution + Mouse liver tissue lysate at 35 µg

    Predicted band size : 57 kDa
  • ab123404 staining IL9R in Mouse Brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C. Samples were incubated with primary antibody (1/300 in PBS + NGS) for 14 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References

ab123404 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Brandon

Verified customer

Submitted Oct 15 2012

DISCOUNT CODES:

Ab10771: **
Ab123404: **

Expiration date: October 28, 2012

Each code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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