The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/12500.
Recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.
Expressed in both lymphoid and non-lymphoid cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling ILF1 with ab50946 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the perinuclear/nuclear membrane. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - ILF1. Right - Merge.
Western blot - ILF1 antibody (ab50946)
Lane 2 : Anti-ILF1 antibody (ab50946) at 0.25 µg/ml
Lane 1 : MARKER Lane 2 : HepG2 cell lysate at 10 µg
Secondary Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution