Overview

  • Product nameAnti-ILF1 antibody
    See all ILF1 primary antibodies
  • Description
    Rabbit polyclonal to ILF1
  • SpecificityThis antibody reacts with ILF1.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A region within synthetic peptide: ANTYTVSGQA VVTPAAVLAP PKAEAQENGD HREVKVKVEP IPAIGHATLG, corresponding to amino acids 491-540 of Human ILF1

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

IHC-P 1/100.

Target

  • FunctionRecognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.
  • Tissue specificityExpressed in both lymphoid and non-lymphoid cells.
  • Sequence similaritiesContains 1 FHA domain.
    Contains 1 fork-head DNA-binding domain.
  • DomainThe C-terminal part of the DNA-binding domain may contribute to DNA recognition specificity.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cellular transcription factor ILF 1 antibody
    • Cellular transcription factor ILF-1 antibody
    • Cellular transcription factor ILF1 antibody
    • Forkhead box K2 antibody
    • Forkhead box protein K2 antibody
    • FOX K1 antibody
    • FOX K2 antibody
    • FOXK 1 antibody
    • FOXK 2 antibody
    • FOXK1 antibody
    • foxk2 antibody
    • FOXK2_HUMAN antibody
    • ILF 1 antibody
    • ILF antibody
    • Interleukin enhancer binding factor 1 antibody
    • Interleukin enhancer-binding factor 1 antibody
    see all

Anti-ILF1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling ILF1 with ab50946 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the perinuclear/nuclear membrane. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - ILF1. Right - Merge.
  • Lane 1 :
    Lane 2 : Anti-ILF1 antibody (ab50946) at 0.25 µg/ml

    Lane 1 : MARKER
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 69 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-ILF1 antibody (ab50946)

ab50946 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab50946.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"