Overview

  • Product name
  • Description
    Rabbit polyclonal to ILF1
  • Specificity
    This antibody reacts with ILF1.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A region within synthetic peptide: ANTYTVSGQA VVTPAAVLAP PKAEAQENGD HREVKVKVEP IPAIGHATLG, corresponding to amino acids 491-540 of Human ILF1

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

IHC-P 1/100.

Target

  • Function
    Recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.
  • Tissue specificity
    Expressed in both lymphoid and non-lymphoid cells.
  • Sequence similarities
    Contains 1 FHA domain.
    Contains 1 fork-head DNA-binding domain.
  • Domain
    The C-terminal part of the DNA-binding domain may contribute to DNA recognition specificity.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cellular transcription factor ILF 1 antibody
    • Cellular transcription factor ILF-1 antibody
    • Cellular transcription factor ILF1 antibody
    • Forkhead box K2 antibody
    • Forkhead box protein K2 antibody
    • FOX K1 antibody
    • FOX K2 antibody
    • FOXK 1 antibody
    • FOXK 2 antibody
    • FOXK1 antibody
    • foxk2 antibody
    • FOXK2_HUMAN antibody
    • ILF 1 antibody
    • ILF antibody
    • Interleukin enhancer binding factor 1 antibody
    • Interleukin enhancer-binding factor 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling ILF1 with ab50946 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the perinuclear/nuclear membrane. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - ILF1. Right - Merge.
  • Lane 1 :
    Lane 2 : Anti-ILF1 antibody (ab50946) at 0.25 µg/ml

    Lane 1 : MARKER
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 69 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References

ab50946 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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