Overview

  • Product nameAnti-ILF1 antibody
    See all ILF1 primary antibodies
  • Description
    Mouse polyclonal to ILF1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 158-257 of Human ILF1. Target sequence used to make the antibody:SSEKREKQEA SESPVKAVQP HISPLTINIP DTMAHLISPL PSPTGTISAA NSCPSSPRGA GSSGYKVGRV MPSDLNLMAD NSQPENEKEA SGGDSPKDDS.

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 69 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionRecognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.
  • Tissue specificityExpressed in both lymphoid and non-lymphoid cells.
  • Sequence similaritiesContains 1 FHA domain.
    Contains 1 fork-head DNA-binding domain.
  • DomainThe C-terminal part of the DNA-binding domain may contribute to DNA recognition specificity.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cellular transcription factor ILF 1 antibody
    • Cellular transcription factor ILF-1 antibody
    • Cellular transcription factor ILF1 antibody
    • Forkhead box K2 antibody
    • Forkhead box protein K2 antibody
    • FOX K1 antibody
    • FOX K2 antibody
    • FOXK 1 antibody
    • FOXK 2 antibody
    • FOXK1 antibody
    • foxk2 antibody
    • FOXK2_HUMAN antibody
    • ILF 1 antibody
    • ILF antibody
    • Interleukin enhancer binding factor 1 antibody
    • Interleukin enhancer-binding factor 1 antibody
    see all

Anti-ILF1 antibody images

  • All lanes : Anti-ILF1 antibody (ab52795) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution

    Predicted band size : 69 kDa

References for Anti-ILF1 antibody (ab52795)

ab52795 has not yet been referenced specifically in any publications.

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