Overview

  • Product nameAnti-ILF1 antibody
    See all ILF1 primary antibodies
  • Description
    Rabbit polyclonal to ILF1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 540-589 (GTASRIIQTA QTTPVQTVTI VQQAPLGQHQ LPIKTVTQNG THVASVPTAV) of human ILF1 (NP_004505).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRecognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.
  • Tissue specificityExpressed in both lymphoid and non-lymphoid cells.
  • Sequence similaritiesContains 1 FHA domain.
    Contains 1 fork-head DNA-binding domain.
  • DomainThe C-terminal part of the DNA-binding domain may contribute to DNA recognition specificity.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cellular transcription factor ILF 1 antibody
    • Cellular transcription factor ILF-1 antibody
    • Cellular transcription factor ILF1 antibody
    • Forkhead box K2 antibody
    • Forkhead box protein K2 antibody
    • FOX K1 antibody
    • FOX K2 antibody
    • FOXK 1 antibody
    • FOXK 2 antibody
    • FOXK1 antibody
    • foxk2 antibody
    • FOXK2_HUMAN antibody
    • ILF 1 antibody
    • ILF antibody
    • Interleukin enhancer binding factor 1 antibody
    • Interleukin enhancer-binding factor 1 antibody
    see all

Anti-ILF1 antibody images

  • All lanes : Anti-ILF1 antibody (ab83286) at 1/2000 dilution

    Lane 1 : Human 293T cell lysate
    Lane 2 : Mouse ES cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-ILF1 antibody (ab83286) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 69 kDa

References for Anti-ILF1 antibody (ab83286)

ab83286 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE gel)
Sample Mouse Cell lysate - whole cell (ES cell)
Specification ES cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 29 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"