Our Abpromise guarantee covers the use of ab28772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.


Anti-ILF2 antibody images

  • ILF2 staining of human kidney sections with ab28772 at 4-8ug/ml. The arrows indicate cells with positive staining (epithelial cells of collecting tubule). Magnification 40X.
  • Anti-ILF2 antibody (ab28772) at 1.25 µg/ml + Jurkat cell lysate

    Predicted band size : 43 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    ILF2 detected in Jurkat cell lysate. An HRP-conjugated anti-rabbit was used as secondary antibody (suggested concentration 1:50000-100000). Gel concentration is 12%.
  • ICC/IF image of ab28772 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28772, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ILF2 antibody (ab28772) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 20 µg
    Lane 4 : Zebrafish liver homogenate at 20 µg
    Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
    Lane 6 : JURKAT (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    Exposure time : 5 minutes
  • All lanes : Anti-ILF2 antibody (ab28772) at 2.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.

    Predicted band size : 43 kDa

References for Anti-ILF2 antibody (ab28772)

This product has been referenced in:
  • Cao D  et al. Combined proteomic-RNAi screen for host factors involved in human hepatitis delta virus replication. RNA 15:1971-9 (2009). Read more (PubMed: 19776158) »

See 1 Publication for this product

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