Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab89100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 95 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 DZF domain.
  • Post-translational
    modifications
    Phosphorylated by RNA-dependent protein kinase (EIF2AK2).
    Methylated by protein arginine N-methyltransferase 1.
    Arg-609 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBTF antibody
    • Double stranded RNA binding protein 76 antibody
    • Double-stranded RNA-binding protein 76 antibody
    • DRBF antibody
    • DRBP76 antibody
    • ILF3 antibody
    • ILF3_HUMAN antibody
    • Interleukin enhancer binding factor 3 antibody
    • Interleukin enhancer-binding factor 3 antibody
    • M phase phosphoprotein 4 antibody
    • M-phase phosphoprotein 4 antibody
    • MPHOSPH4 antibody
    • MPP4 antibody
    • NF AT 90 antibody
    • NF-AT-90 antibody
    • NF110 antibody
    • NF90 antibody
    • NFAR antibody
    • Nuclear factor associated with dsRNA antibody
    • Nuclear factor of activated T cells 90 kDa antibody
    • Nuclear factor of activated T-cells 90 kDa antibody
    • TCP80 antibody
    • Translational control protein 80 antibody
    see all

Anti-ILF3 antibody images

  • Anti-ILF3 antibody (ab89100) at 1 µg/ml + Human pancreas lysate at 50 µg

    Predicted band size : 95 kDa
    Observed band size : 80,95 kDa (why is the actual band size different from the predicted?)
  • Anti-ILF3 antibody (ab89100) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 95 kDa
    Observed band size : 95 kDa
    Additional bands at : 105 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-ILF3 antibody (ab89100) at 1 µg/ml

    Lane 1 : 293T transfected cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 95 kDa
    Observed band size : 95 kDa
  • ab89100, at 3µg/ml, staining human ILF3 in small intestine, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
  • Immunofluorescence of ab89100, used at 10µg/ml, to ILF3 on HeLa cells.

References for Anti-ILF3 antibody (ab89100)

ab89100 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
85 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Primary macrophage)
Specification
Primary macrophage
Blocking step
Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. David Gregory

Verified customer

Submitted Mar 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up