Anti-ILF3 antibody (ab89100)
Key features and details
- Mouse polyclonal to ILF3
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ILF3 antibody
See all ILF3 primary antibodies -
Description
Mouse polyclonal to ILF3 -
Host species
Mouse -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human ILF3 aa 1-750. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_004507 -
Positive control
- WB: Human pancreas or HeLa nuclear lysate. IHC-P: Human small intestine tissue. ICC: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 10 µg/ml.
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 95 kDa.
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IHC-P |
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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Notes |
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ICC
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 95 kDa. |
IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
Target
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Function
May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 DZF domain. -
Post-translational
modificationsPhosphorylated by RNA-dependent protein kinase (EIF2AK2).
Methylated by protein arginine N-methyltransferase 1.
Arg-609 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 3609 Human
- Omim: 603182 Human
- SwissProt: Q12906 Human
- Unigene: 465885 Human
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Alternative names
- CBTF antibody
- Double stranded RNA binding protein 76 antibody
- Double-stranded RNA-binding protein 76 antibody
see all
Images
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Anti-ILF3 antibody (ab89100) at 1 µg/ml + Human pancreas lysate at 50 µg
Predicted band size: 95 kDa
Observed band size: 80,95 kDa why is the actual band size different from the predicted? -
Anti-ILF3 antibody (ab89100) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 95 kDa
Observed band size: 95 kDa
Additional bands at: 105 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-ILF3 antibody (ab89100) at 1 µg/ml
Lane 1 : 293T transfected cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 95 kDa
Observed band size: 95 kDa -
ab89100, at 3µg/ml, staining human ILF3in small intestine, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
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Immunocytochemistry analysis of HeLa cells labeling ILF3 with ab89100 at 10 µg/mL.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab89100 has been referenced in 2 publications.
- Liu Y et al. Upregulation of ERp57 promotes clear cell renal cell carcinoma progression by initiating a STAT3/ILF3 feedback loop. J Exp Clin Cancer Res 38:439 (2019). PubMed: 31747963
- Zhuang Q et al. Long noncoding RNA lncAIS downregulation in mesenchymal stem cells is implicated in the pathogenesis of adolescent idiopathic scoliosis. Cell Death Differ 26:1700-1715 (2019). PubMed: 30464226