Overview

  • Product nameAnti-ILKAP antibody
    See all ILKAP primary antibodies
  • Description
    Rabbit polyclonal to ILKAP
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    synthesized peptide derived from the internal of human ILKAP.

  • Positive control
    • Extracts from COS7 cells. Colon carcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • FunctionProtein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.
  • Tissue specificityWidely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.
  • Sequence similaritiesBelongs to the PP2C family.
    Contains 1 PP2C-like domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434J2031 antibody
    • FLJ10181 antibody
    • ILKAP antibody
    • ILKAP_HUMAN antibody
    • ILKAP2 antibody
    • ILKAP3 antibody
    • Integrin linked kinase associated phosphatase antibody
    • Integrin linked kinase associated serine/threonine phosphatase 2C antibody
    • Integrin linked kinase associated serine/threonine phosphatase antibody
    • Integrin-linked kinase-associated serine/threonine phosphatase 2C antibody
    • MGC4846 antibody
    • PP2C DELTA antibody
    • Protein phosphatase 2c delta isozyme antibody
    see all

Anti-ILKAP antibody images

  • All lanes : Anti-ILKAP antibody (ab70019)

    Lane 1 : extracts from COS7 cells
    Lane 2 : extracts from COS7 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 36 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 1/50 ab70019. Right: with antibody pre-treatment with immunizing peptide

References for Anti-ILKAP antibody (ab70019)

ab70019 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"