Overview

  • Product name
    Anti-ILP2 antibody
  • Description
    Rabbit polyclonal to ILP2
  • Tested applications
    Suitable for: ICC/IF, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    TGYEARLITFGT

    , corresponding to amino acids 2-13 of Human ILP2. (Peptide available as ab9665.)

  • Positive control
    • Human HepG2 or MOLT4 cell lysate
  • General notes


    Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2 (1). ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues (1). ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2 (1). ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues (1). ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 33 kDa.Can be blocked with Human ILP2 peptide (ab9665).
ICC Use a concentration of 10 µg/ml.

Target

  • Function
    Protects against apoptosis mediated by BAX.
  • Tissue specificity
    Testis specific in normal tissues.
  • Sequence similarities
    Belongs to the IAP family.
    Contains 1 BIR repeat.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Baculoviral IAP repeat containing 8 antibody
    • Baculoviral IAP repeat containing protein 8 antibody
    • Baculoviral IAP repeat-containing protein 8 antibody
    • BIRC 8 antibody
    • BIRC8 antibody
    • BIRC8 protein antibody
    • BIRC8_HUMAN antibody
    • hILP 2 antibody
    • hILP2 antibody
    • IAP like protein 2 antibody
    • IAP-like protein 2 antibody
    • ILP 2 antibody
    • ILP-2 antibody
    • Inhibitor of apoptosis like protein 2 antibody
    • Inhibitor of apoptosis-like protein 2 antibody
    • Testis specific inhibitor of apoptosis antibody
    • Testis-specific inhibitor of apoptosis antibody
    see all

Images

  • Western blot analysis of ILP-2 expression in human HepG2 (lane 1) and MOLT4 (lane 2) cell lysates and mouse spleen (lane 3) tissue lysates with anti-ILP-2 at 1 µg/ml.
  • ab9664 at 10µg/ml staining ILP2 in HepG2 cells by Immunocytochemistry.

References

ab9664 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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