Overview

  • Product name
    Iminodyn™ 17
  • Description
    Negative control for Iminodyn™ 22
  • Biological description
    Negative control for Iminodyn™ 22, for use in cell based assays. Potently inhibits dynamin I and II GTPase activity in vitro (IC50 values are 330 and 440 nM, respectively), but does not affect dynamin-mediated endocytosis in cells (IC50 values are ≥300 μM for receptor-mediated and synaptic vesicle endocytosis).
  • Purity
    > 98%
  • General notes
    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Iminodyn™ is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.

Properties

Applications

Our Abpromise guarantee covers the use of ab120462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab66705 staining PAI1 in HeLa cells treated with iminodyn 17™ (ab120462), by ICC/IF. No change in PAI1 expression was observed with incubation with iminodyn 17™, as described in literature.
    The cells were incubated at 37°C for 48h in media containing different concentrations of ab120462 (Iminodyn-17™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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