Overview

  • Product nameIminodyn™ 22
  • Description
    Potent, broad spectrum dynamin inhibitor
  • Biological descriptionPotent, broad spectrum dynamin inhibitor. (IC50 values are 450 and 390 nM for inhibition of dynamin I and II GTPase, respectively). Binds to the GTPase domain at an allosteric site and displays uncompetitive antagonism with respect to GTP. Inhibits receptor mediated endocytosis (RME) and synaptic vesicle endocytosis (SVE) (IC50 values are 10.7 and 108 μM, respectively).
  • Purity> 98%
  • General notesSold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Iminodyn™ is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120461 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Iminodyn™ 22 images

    • ab66705 staining PAI1 in HeLa cells treated with iminodyn-22™ (ab120461), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of iminodyn-22™, as described in literature.
      The cells were incubated at 37°C for 48h in media containing different concentrations of ab120461 (Iminodyn-22™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Iminodyn™ 22 (ab120461)

    This product has been referenced in:

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    Product Wall

    Thank you for contacting us. I apologize for the confusion regarding extinction coefficient and IC50.

    Unfortunately we do not have the molar extinction coefficient for this compound. However, our solubility information for this compound is giv...

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    Thank you for contacting us.

    Are you interested in knowing the IC50 value or EC50 value?

    The IC50 values are10.7 and 108 μM respectively and these are cited on datasheet.

    I hope this information is helpful to you. Please d...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"