Overview

  • Product name
    Anti-IMP3 antibody
    See all IMP3 primary antibodies
  • Description
    Goat polyclonal to IMP3
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-QRREDYTRYNQLSR

    , corresponding to internal sequence amino acids 40-53 of Human IMP3 (NP_060755.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human Liver lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab105022 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Target

Images

  • Western blot - IMP3 antibody (ab105022)
    Western blot - IMP3 antibody (ab105022)
    Anti-IMP3 antibody (ab105022) at 0.05 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 22 kDa



    Primary incubation was 1 hour.

References

ab105022 has not yet been referenced specifically in any publications.

Publishing research using ab105022? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

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I used the anti-IMP3 antibody (ab105022) in western blots using pancreatic cyst fluid as the sample. As a positive control, I used a recombinant IMP3 protein (GST tagged). I have attached the gel in this email. Lane 1 is the molecular weight ladder; La...

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Thank you for sending your data.

Can you please tell me how the samples are prepared for loading into the gel? Are these reduced and denatured?

I suspect that the multiple bands in the lanes containing the cyst fluid samples are m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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