Overview

  • Product nameAnti-IMP3 antibody, prediluted
    See all IMP3 primary antibodies
  • Description
    Rabbit polyclonal to IMP3, prediluted
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human IMP3 (internal sequence).

  • Positive control
    • Human placenta.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab82044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
READY TO USE.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionRNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA.
  • Tissue specificityExpressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.
  • Sequence similaritiesBelongs to the RRM IMP/VICKZ family.
    Contains 4 KH domains.
    Contains 2 RRM (RNA recognition motif) domains.
  • DomainThe third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
  • Cellular localizationNucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 98 antibody
    • CT98 antibody
    • DKFZp686F1078 antibody
    • hKOC antibody
    • IF2B3_HUMAN antibody
    • IGF II mRNA binding protein 3 antibody
    • IGF-II mRNA-binding protein 3 antibody
    • IGF2 mRNA binding protein 3 antibody
    • IGF2 mRNA-binding protein 3 antibody
    • IGF2BP3 antibody
    • IMP 3 antibody
    • IMP-3 antibody
    • Insulin like growth factor 2 mRNA binding protein 3 antibody
    • Insulin-like growth factor 2 mRNA-binding protein 3 antibody
    • KH domain containing protein overexpressed in cancer antibody
    • KH domain-containing protein overexpressed in cancer antibody
    • KOC 1 antibody
    • KOC1 antibody
    • VICKZ 3 antibody
    • VICKZ family member 3 antibody
    • VICKZ3 antibody
    see all

Anti-IMP3 antibody, prediluted images

  • ab82044, prediluted, staining IMP3 in formalin fixed, paraffin embedded human placenta tissue by Immunohistochemistry.
  • ICC/IF image of ab82044 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82044, neat) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-IMP3 antibody, prediluted (ab82044)

This product has been referenced in:
  • Wang J  et al. E-Cadherin, CD44v6, and Insulin-Like Growth Factor-II mRNA-Binding Protein 3 Expressions in Different Stages of Hydatidiform Moles. J Biochem Mol Toxicol 30:455-61 (2016). Read more (PubMed: 27238915) »

See 1 Publication for this product

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Thank you for your reply and for providing further information.

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Thanks for your email. ab4587 does not have any required MSDS information, so a link will not be present on this datasheet. For example, ab7254 does have a link to MSDS information. I hope this helps. Please contact me again if you have any further que...

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Thanks for your enquiry. If required, MSDS information is located via one or more 'MSDS' links near the top left corner of the online datasheet. This link is just below the 'Datasheet PDF' link present on all datasheets. I hope this is helpful. Please ...

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Thank you for your enquiry. Unfortunately the immunogen sequence information is proprietary. However, I can provide a 50 amino acid sequence from where the sequence was taken from. A LKAIEALSGK IELHGKPIEV EHSVPKRQRI RKLQIRNIPP HLQWEVLDSL (50-...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"