Overview

  • Product nameAnti-IMPA1 antibody
    See all IMPA1 primary antibodies
  • Description
    Rabbit polyclonal to IMPA1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human IMPA1.

    (Peptide available as ab74969.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Liver (Mouse) Tissue, Testis (Human) Tissue, SK N SH Whole Cell, Jurkat Whole Cell, Thymus (Rat) Tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab73074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionResponsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Can use myo-inositol monophosphates, myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates.
  • PathwayPolyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.
  • Sequence similaritiesBelongs to the inositol monophosphatase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • IMP 1 antibody
    • IMP antibody
    • IMPA 1 antibody
    • IMPA antibody
    • IMPA1 antibody
    • IMPA1_HUMAN antibody
    • IMPase 1 antibody
    • IMPase antibody
    • Inositol 1(or 4) monophosphatase antibody
    • Inositol monophosphatase 1 antibody
    • Inositol monophosphatase antibody
    • Inositol(myo) 1(or 4) monophosphatase 1 antibody
    • Inositol-1(or 4)-monophosphatase 1 antibody
    • Lithium sensitive myo inositol monophosphatase A1 antibody
    • Lithium-sensitive myo-inositol monophosphatase A1 antibody
    see all

Anti-IMPA1 antibody images

  • All lanes : Anti-IMPA1 antibody (ab73074) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : Thymus (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 49 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab73074 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73074, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.

References for Anti-IMPA1 antibody (ab73074)

ab73074 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Spinal cord)
Specification Spinal cord
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

Submitted Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"