Recombinant
RabMAb

Anti-IMPA1 [EPR15405] antibody (ab191570)

Overview

  • Product name
    Anti-IMPA1 [EPR15405] antibody
    See all IMPA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR15405] to IMPA1
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human IMPA1 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P29218

  • Positive control
    • Human testis lysate; Jurkat; PC3 and HCT 116 cell lysates; Mouse brain lysate; PC3 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab191570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/300. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/10000 - 1/100000. Predicted molecular weight: 30 kDa.

Target

  • Function
    Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Can use myo-inositol monophosphates, myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates.
  • Pathway
    Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.
  • Sequence similarities
    Belongs to the inositol monophosphatase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • IMP 1 antibody
    • IMP antibody
    • IMPA 1 antibody
    • IMPA antibody
    • IMPA1 antibody
    • IMPA1_HUMAN antibody
    • IMPase 1 antibody
    • IMPase antibody
    • Inositol 1(or 4) monophosphatase antibody
    • Inositol monophosphatase 1 antibody
    • Inositol monophosphatase antibody
    • Inositol(myo) 1(or 4) monophosphatase 1 antibody
    • Inositol-1(or 4)-monophosphatase 1 antibody
    • Lithium sensitive myo inositol monophosphatase A1 antibody
    • Lithium-sensitive myo-inositol monophosphatase A1 antibody
    see all

Images

  • Flow cytometric analysis of PC3 cells labeling IMPA1 using ab191570at 1/300 dilution (red), Isotype control (green). Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Cells were fixed in 2% paraformaldehyde prior to staining.

  • Anti-IMPA1 [EPR15405] antibody (ab191570) at 1/20000 dilution + Mouse brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 30 kDa

    5% NFDM/TBST blocking buffer

  • All lanes : Anti-IMPA1 [EPR15405] antibody (ab191570) at 1/20000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : PC3 cell lysate
    Lane 3 : HCT 116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 30 kDa

    5% NFDM/TBST blocking buffer

  • Anti-IMPA1 [EPR15405] antibody (ab191570) at 1/100000 dilution + Human testis lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 30 kDa

    5% NFDM/TBST blocking buffer

References

ab191570 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191570.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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