Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis.
Widely expressed. Expressed at high level in brain.
Belongs to the IMPACT family. Contains 1 RWD domain.
Imprinted and ancient gene protein homolog antibody
Protein IMPACT antibody
RWD domain containing 5 antibody
Western blot - IMPACT antibody (ab95175)
All lanes : Anti-IMPACT antibody (ab95175) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 36 kDa
Exposure time : 30 seconds
Immunoprecipitation - IMPACT antibody (ab95175)
Detection of IMPACT in immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab95175 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.