Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis.
Widely expressed. Expressed at high level in brain.
Belongs to the IMPACT family. Contains 1 RWD domain.
Detection of IMPACT in immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab95175 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-IMPACT antibody (ab95175) at 0.4 µg/ml
Lane 1 : IP with IMPACT antibody Lane 2 : IP with ab95175 Lane 3 : IP with control IgG