• Product nameAnti-IMPDH1 antibody
    See all IMPDH1 primary antibodies
  • Description
    Rabbit polyclonal to IMPDH1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (RSKKGKLPIV NDCDELVAII ARTDLKKNRD YPLASKDSQK QLLCGAAVGT) of Human IMPDH1 (NP_899066).

  • Positive control
    • HeLa cell lysate.



Our Abpromise guarantee covers the use of ab84957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.


  • FunctionRate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
  • Tissue specificityIMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
  • PathwayPurine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
  • Involvement in diseaseDefects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10) [MIM:180105]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant.
  • Sequence similaritiesBelongs to the IMPDH/GMPR family.
    Contains 2 CBS domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • IMDH1_HUMAN antibody
    • IMP (inosine monophosphate) dehydrogenase 1 antibody
    • IMP dehydrogenase 1 antibody
    • IMPD 1 antibody
    • IMPD antibody
    • IMPD1 antibody
    • IMPDH 1 antibody
    • IMPDH I antibody
    • IMPDH-I antibody
    • Impdh1 antibody
    • Inosine 5' monophosphate dehydrogenase 1 antibody
    • Inosine monophosphate dehydrogenase 1 antibody
    • Inosine-5''-monophosphate dehydrogenase 1 antibody
    • LCA11 antibody
    • RP10 antibody
    • sWSS2608 antibody
    see all

Anti-IMPDH1 antibody images

  • Anti-IMPDH1 antibody (ab84957) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 27 kDa,31 kDa. We are unsure as to the identity of these extra bands.
  • ab84957 staining IMPDH1 in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab84957 staining IMPDH1 in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-IMPDH1 antibody (ab84957)

ab84957 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human cancer xenograft)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH 9
Permeabilization No
Specification human cancer xenograft
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Formaldehyde

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Submitted Jun 24 2015