Recombinant Anti-IMPDH2 + IMPDH1 antibody [EPR9678(B)] (ab137120)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9678(B)] to IMPDH2 + IMPDH1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IMPDH2 + IMPDH1 antibody [EPR9678(B)] -
Description
Rabbit monoclonal [EPR9678(B)] to IMPDH2 + IMPDH1 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 92% homology with IPMDH2. Cross-reactivity with this protein has not been confirmed experimentally. -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: IMPDH1 and IMPDH2 recombinant protein, HeLa, Jurkat, K562, and Raji cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9678(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
Images
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All lanes : Anti-IMPDH2 + IMPDH1 antibody [EPR9678(B)] (ab137120) at 1/1000 dilution
Lane 1 : DDK tagged human full length IMPDH1 recombinant protein (P20839-3), 10ng
Lane 2 : DDK tagged human full length IMPDH2 recombinant protein (P12268), 10ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55,60 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-IMPDH2 + IMPDH1 antibody [EPR9678(B)] (ab137120) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab137120 has been referenced in 1 publication.
- Valvezan AJ et al. mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability. Cancer Cell 32:624-638.e5 (2017). PubMed: 29056426