Recombinant Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] (ab137112)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9679(B)] to IMPDH2 + IMPDH1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] -
Description
Rabbit monoclonal [EPR9679(B)] to IMPDH2 + IMPDH1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat and K562 cell, C-Myc/DDK tagged full length IMPDH1 and IMPDH2 Human Recombinant Protein lysates FC (intra): K562 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9679(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa. |
Images
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All lanes : Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] (ab137112) at 1/1000 dilution
Lane 1 : C-Myc/DDK tagged full length IMPDH1 Human Recombinant Protein
Lane 2 : C-Myc/DDK tagged full length IMPDH2 Human Recombinant Protein
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Intracellular flow cytometric analysis of permeabilized K562 cells labelling IMPDH1 with ab137112 (red) at 1/500 dilution or a rabbit IgG (negative) (green).
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All lanes : Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] (ab137112) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP-labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137112 has not yet been referenced specifically in any publications.