The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionRate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
Tissue specificityIMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
PathwayPurine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Sequence similaritiesBelongs to the IMPDH/GMPR family. Contains 2 CBS domains.
Post-translational modificationsThe N-terminus is blocked.
IHC image of ab75790 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75790, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab75790 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75790, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody (ab75790)Image from Carcamo WC et al., PLoS One. 2011;6(12):e29690. Epub 2011 Dec 29. Fig 4.; doi:10.1371/journal.pone.0029690; December 29, 2011, PLoS ONE 6(12): e29690.
ab75790 staining IMPDH2 in HEp-2 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody against IMPDH2 (red) and nuclei were stained with DAPI (blue).
References for Anti-IMPDH2 antibody (ab75790)
This product has been referenced in:
Fromm-Dornieden C et al. Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells. BMC Mol Biol13:9 (2012).
Read more (PubMed: 22436005) »
Carcamo WC et al. Induction of cytoplasmic rods and rings structures by inhibition of the CTP and GTP synthetic pathway in mammalian cells. PLoS One6:e29690 (2011).
Read more (PubMed: 22220215) »