The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
FunctionRate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
Tissue specificityIMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
PathwayPurine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Sequence similaritiesBelongs to the IMPDH/GMPR family. Contains 2 CBS domains.
Post-translational modificationsThe N-terminus is blocked.