Recombinant Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8364(B)] to IMPDH2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IMPDH2 antibody [EPR8364(B)]
See all IMPDH2 primary antibodies -
Description
Rabbit monoclonal [EPR8364(B)] to IMPDH2 -
Host species
Rabbit -
Specificity
The immunogen used to raise this antibody shares 79% homology with Human IMPDH1.
This antibody shows very faint band when testing IMPDH1 recombinant protein in WB test.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human IMPDH2 aa 100-200. The exact sequence is proprietary.
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Positive control
- K562, HepG2, HeLa and NCI-H460 cell lysates; Human colon tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8364(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
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IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. -
Tissue specificity
IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor. -
Pathway
Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1. -
Sequence similarities
Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains. -
Post-translational
modificationsThe N-terminus is blocked. - Information by UniProt
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Database links
- Entrez Gene: 3615 Human
- Entrez Gene: 23918 Mouse
- Entrez Gene: 301005 Rat
- Omim: 146691 Human
- SwissProt: P12268 Human
- SwissProt: P24547 Mouse
- SwissProt: E9PU28 Rat
- Unigene: 654400 Human
see all -
Alternative names
- IMDH2_HUMAN antibody
- IMP (inosine monophosphate) dehydrogenase 2 antibody
- IMP dehydrogenase 2 antibody
see all
Images
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ab131158 staining IMPDH2 in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.
DAPI was used as a nuclear counterstain and the negative control was PBS only.
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All lanes : Anti-IMPDH2 antibody [EPR8364(B)] (ab131158) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NCI-H460 cell lysate
Predicted band size: 56 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IMPDH2 with ab131158 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-IMPDH2 antibody [EPR8364(B)] (ab131158) at 1/1000 dilution
Lanes 1 & 3 : C-Myc/DDK tagged full length IMPDH1 Human Recombinant Protein
Lane 2 : C-Myc/DDK tagged full length IMPDH2 Human Recombinant Protein
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times:Lane 1&2: 5 seconds
Lane 3: 180 seconds.
This antibody shows very faint band when testing IMPDH1 recombinant protein in WB test
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab131158 has been referenced in 13 publications.
- He S et al. A recombinant scFv antibody-based fusion protein that targets EGFR associated with IMPDH2 downregulation and its drug conjugate show therapeutic efficacy against esophageal cancer. Drug Deliv 29:1243-1256 (2022). PubMed: 35416106
- Chaiyawat P et al. IMPDH2 and HPRT expression and a prognostic significance in preoperative and postoperative patients with osteosarcoma. Sci Rep 11:10887 (2021). PubMed: 34035425
- Xu H et al. IMPDH2 promotes cell proliferation and epithelial-mesenchymal transition of non-small cell lung cancer by activating the Wnt/ß-catenin signaling pathway. Oncol Lett 20:219 (2020). PubMed: 32963625
- Kofuji S et al. IMP dehydrogenase-2 drives aberrant nucleolar activity and promotes tumorigenesis in glioblastoma. Nat Cell Biol 21:1003-1014 (2019). PubMed: 31371825
- Wolfe K et al. Dynamic compartmentalization of purine nucleotide metabolic enzymes at leading edge in highly motile renal cell carcinoma. Biochem Biophys Res Commun 516:50-56 (2019). PubMed: 31196624