Anti-Importin 13 antibody (ab101374)
Key features and details
- Rabbit polyclonal to Importin 13
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Importin 13 antibody
See all Importin 13 primary antibodies -
Description
Rabbit polyclonal to Importin 13 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 143-280 of Human Importin 13 (AAH08194)
-
Positive control
- A549, HeLa, HepG2 and HCT116 cells. MCF7 xenograft.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 108 kDa.
|
|
IHC-P |
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer pH 8.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 108 kDa. |
IHC-P
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer pH 8. |
Target
-
Function
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13. -
Tissue specificity
Expressed in fetal brain, heart, intestine and kidney. -
Sequence similarities
Belongs to the importin beta family.
Contains 14 HEAT repeats.
Contains 1 importin N-terminal domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 523399 Cow
- Entrez Gene: 9670 Human
- Entrez Gene: 230673 Mouse
- Entrez Gene: 116458 Rat
- GenBank: NM_014652 Human
- Omim: 610411 Human
- SwissProt: A7YWD2 Cow
- SwissProt: O94829 Human
see all -
Alternative names
- Imp 13 antibody
- Imp13 antibody
- Importin-13 antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab101374 has been referenced in 1 publication.
- Choi HK et al. Programmed cell death 5 mediates HDAC3 decay to promote genotoxic stress response. Nat Commun 6:7390 (2015). PubMed: 26077467