Overview

  • Product name
  • Description
    Rabbit polyclonal to Importin 13
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide containing a sequence corresponding to a region within amino acids 901-963 (SMWIKEALQP PGFPSARLSP EQKDTFSQQI LRERVNKRRV KEMVKEFTLL CRGLHGTDYT ADY) of Human Importin 13 (NP_055467).

  • Positive control
    • HeLa whole cell lysate; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab95993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 108 kDa.
IP Use at 10-1 µg/mg of lysate.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13.
  • Tissue specificity
    Expressed in fetal brain, heart, intestine and kidney.
  • Sequence similarities
    Belongs to the importin beta family.
    Contains 14 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Imp 13 antibody
    • Imp13 antibody
    • Importin-13 antibody
    • Importin13 antibody
    • IPO 13 antibody
    • Ipo13 antibody
    • IPO13_HUMAN antibody
    • Kap 13 antibody
    • Kap13 antibody
    • Karyopherin 13 antibody
    • Karyopherin-13 antibody
    • Karyopherin13 antibody
    • KIAA0274 antibody
    • Late gestation lung 2 antibody
    • Late gestation lung 2 protein antibody
    • Lgl2 antibody
    • Ran binding protein 13 antibody
    • Ran-binding protein 13 antibody
    • RanBP 13 antibody
    • RanBP13 antibody
    see all

Images

  • All lanes : Anti-Importin 13 antibody (ab95993) at 500

    Lane 1 : Non-transfected lysate
    Lane 2 : IPO13 transfected lysate


    Predicted band size : 108 kDa
  • Importin 13 was immunoprecipitated from 1 mg of HeLa whole cell lysate using 10 µg of ab95993. The precipitated Importin 13 was detected with ab95993 at 1/1000 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab95993 at 1/200 dilution. Lower image is merged with DNA probe.

References

This product has been referenced in:
  • Xue J  et al. Forkhead Box M1 Is Essential for Nuclear Localization of Glioma-associated Oncogene Homolog 1 in Glioblastoma Multiforme Cells by Promoting Importin-7 Expression. J Biol Chem 290:18662-70 (2015). Read more (PubMed: 26085085) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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