Overview

  • Product nameAnti-Importin 7 antibody
    See all Importin 7 primary antibodies
  • Description
    Goat polyclonal to Importin 7
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog
  • Immunogen

    Synthetic peptide:

    SSFNFGGPAPGMN

    with a Cysteine residue linker, corresponding to C terminal amino acids 1026 - 1038 of Human Importin 7.

  • Positive control
    • Human breast tissue; Human brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 120 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionFunctions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In vitro, mediates the nuclear import of HIV-1 reverse transcription complex (RTC) integrase. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
  • Sequence similaritiesBelongs to the importin beta family.
    Contains 1 importin N-terminal domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ14581 antibody
    • Imp 7 antibody
    • Imp7 antibody
    • Importin-7 antibody
    • Importin7 antibody
    • IPO 7 antibody
    • IPO7 antibody
    • IPO7_HUMAN antibody
    • MGC138673 antibody
    • Ran binding protein 7 antibody
    • Ran-binding protein 7 antibody
    • RANBP 7 antibody
    • RanBP7 antibody
    see all

Anti-Importin 7 antibody images

  • ab115208 at 5 ug/ml, staining Importin 7 in Formalin-fixed, Paraffin-embedded Human breast tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-Importin 7 antibody (ab115208) at 0.1 µg/ml + Human brain lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 120 kDa

References for Anti-Importin 7 antibody (ab115208)

ab115208 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"