Overview

  • Product nameAnti-Importin 7 antibody
    See all Importin 7 primary antibodies
  • Description
    Rabbit polyclonal to Importin 7
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Elephant
  • Immunogen

    Synthetic peptide within Human Importin 7 aa 200-250. The exact sequence is proprietary.
    Database link: NP_006382.1

  • Positive control
    • HeLa whole cell lysate, 293T whole cell lysate and Mouse NIH3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab99273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 120 kDa.
IP Use at 5-15 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionFunctions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In vitro, mediates the nuclear import of HIV-1 reverse transcription complex (RTC) integrase. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
  • Sequence similaritiesBelongs to the importin beta family.
    Contains 1 importin N-terminal domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ14581 antibody
    • Imp 7 antibody
    • Imp7 antibody
    • Importin-7 antibody
    • Importin7 antibody
    • IPO 7 antibody
    • IPO7 antibody
    • IPO7_HUMAN antibody
    • MGC138673 antibody
    • Ran binding protein 7 antibody
    • Ran-binding protein 7 antibody
    • RANBP 7 antibody
    • RanBP7 antibody
    see all

Anti-Importin 7 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Importin 7 with ab99273 at 1/1000 (0.2µg/mg). Detection: DAB.
  • All lanes : Anti-Importin 7 antibody (ab99273) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 120 kDa


    Exposure time : 3 seconds
  • ab99273 at 0.1 µg/ml detecting Importin 7 in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 1 second exposure.
    For immunoprecipitation, ab99273 was used at at 10 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99272 which recognizes an upstream epitope of Importin 7.
    Lane 2; IP using ab99273.
    Lane 3; IP using control IgG.

References for Anti-Importin 7 antibody (ab99273)

This product has been referenced in:
  • Wei Y  et al. Importin 8 regulates the transport of mature microRNAs into the cell nucleus. J Biol Chem 289:10270-5 (2014). Mouse . Read more (PubMed: 24596094) »

See 1 Publication for this product

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