Overview

  • Product nameAnti-Importin 8 antibody
    See all Importin 8 primary antibodies
  • Description
    Goat polyclonal to Importin 8
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: SAFNFGTVPSNN, corresponding to C terminal amino acids 1026-1037 of Human Importin 8.

  • General notes


    The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notesThe importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab5263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent dilution. Can be blocked with Human Importin 8 peptide (ab23081). Preliminary experiments gave an approx 23kDa band in lysates of cell lines A431, A549 and HeLa after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 120kDa.

Target

  • FunctionSeems to function in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of SRP19.
  • Sequence similaritiesBelongs to the importin beta family.
    Contains 1 importin N-terminal domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ26580 antibody
    • Imp 8 antibody
    • Imp8 antibody
    • Importin-8 antibody
    • Importin8 antibody
    • IPO 8 antibody
    • IPO8 antibody
    • IPO8_HUMAN antibody
    • Ran binding protein 8 antibody
    • RAN BP8 antibody
    • Ran-binding protein 8 antibody
    • RANBP 8 antibody
    • RanBP8 antibody
    see all

Anti-Importin 8 antibody images

  • ab5263 (4µg/ml) staining of paraffin embedded Human Tonsil shows cytoplam staining in the parenchyma. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

References for Anti-Importin 8 antibody (ab5263)

ab5263 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"