Anti-Importin 8 antibody (ab72109)

Overview

  • Product nameAnti-Importin 8 antibody
    See all Importin 8 primary antibodies
  • Description
    Rabbit polyclonal to Importin 8
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Chimpanzee, Monkey
    Predicted to work with: Mouse, Chicken, Cow, Dog
  • Immunogen

    A portion of amino acids 850-900 of human Importin 8

  • Positive control
    • HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab72109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 120 kDa).
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • FunctionSeems to function in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of SRP19.
  • Sequence similaritiesBelongs to the importin beta family.
    Contains 1 importin N-terminal domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ26580 antibody
    • Imp 8 antibody
    • Imp8 antibody
    • Importin-8 antibody
    • Importin8 antibody
    • IPO 8 antibody
    • IPO8 antibody
    • IPO8_HUMAN antibody
    • Ran binding protein 8 antibody
    • RAN BP8 antibody
    • Ran-binding protein 8 antibody
    • RANBP 8 antibody
    • RanBP8 antibody
    see all

Anti-Importin 8 antibody images

  • All lanes : Anti-Importin 8 antibody (ab72109) at 1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate with immunizing peptide


    Predicted band size : 120 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • Staining of HeLa cells using ab72109 (left) or a control rabbit Ig (right) at 5ug/ml.

References for Anti-Importin 8 antibody (ab72109)

ab72109 has not yet been referenced specifically in any publications.

Product Wall

Thank you for this link. So as I understand it, our target for this product is Importin 8, which is different from Importin alpha 8. Importin alpha 8 has an alternative name of KPNA7 and is ˜ 56 kD, while importin 8 iteself is a larger protein ˜ 120 kD...

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