Recombinant
RabMAb

Anti-Importin 9 antibody [EPR1352] (ab124710)

Overview

  • Product name
    Anti-Importin 9 antibody [EPR1352]
    See all Importin 9 primary antibodies
  • Description
    Rabbit monoclonal [EPR1352] to Importin 9
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Importin 9 protein (Q96P70).

  • Positive control
    • HeLa, SKBR-3, C6, and NIH/3T3 cell lysates and Human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 116 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.
    • Sequence similarities
      Belongs to the importin beta family.
      Contains 1 importin N-terminal domain.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Imp9 antibody
      • Imp9a antibody
      • Imp9b antibody
      • Importin-9 antibody
      • Importin-9a antibody
      • Ipo9 antibody
      • IPO9_HUMAN antibody
      • Ran-binding protein 9 antibody
      • RanBP9 antibody
      see all

    Images

    • All lanes : Anti-Importin 9 antibody [EPR1352] (ab124710) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SKBR-3 cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 116 kDa
    • ab124710, at 1/500 to 1/1000, staining Impotin 9 in Human brain tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124710 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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