Overview

  • Product nameAnti-Importin4 antibody
    See all Importin4 primary antibodies
  • Description
    Rabbit polyclonal to Importin4
  • Tested applicationsSuitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 900-950 (LLPVLLSTAQ EADPEVRSNA IFGMGVLAEH GGHPAQEHFP KLLGLLFPLL A) of Human Importin4 (NP_078934.3).

  • Positive control
    • HeLa whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab99271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 5-15 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionFunctions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
    • Sequence similaritiesBelongs to the importin beta family.
      Contains 6 HEAT repeats.
      Contains 1 importin N-terminal domain.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Imp4 antibody
      • Imp4b antibody
      • Importin 4 antibody
      • Importin 4b antibody
      • Importin-4 antibody
      • Importin-4b antibody
      • Ipo4 antibody
      • IPO4_HUMAN antibody
      • Ran binding protein 4 antibody
      • Ran-binding protein 4 antibody
      • RanBP4 antibody
      see all

    Anti-Importin4 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Importin4 with ab99271 at 1/5000 (0.2µg/mg). Detection: DAB.
    • Detection of Importin4 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99271 at 10 µg/mg lysate for IP. ab99270, which recognizes an upstream epitope, was used at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 5 seconds.

    References for Anti-Importin4 antibody (ab99271)

    This product has been referenced in:
    • Wei Y  et al. Importin 8 regulates the transport of mature microRNAs into the cell nucleus. J Biol Chem 289:10270-5 (2014). IP ; Mouse . Read more (PubMed: 24596094) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"