In situ BrdU-Red DNA Fragmentation (TUNEL) Assay Kit (ab66110)
- Product nameIn situ BrdU-Red DNA Fragmentation (TUNEL) Assay KitSee all DNA fragments kits ...
- Tests60 x 1 assay
- Sample typeTissue, Adherent cells, Suspension cells
- Assay typeQuantitative
- Assay time3h 00m
- Product overview
Abcam's In situ BrdU-Red DNA Fragmentation (TUNEL) Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a Red fluorescence labeled anti-BrdU monoclonal antibody. The assay is suitable for studying apoptosis with GFP transfected cells.
Visit our FAQs page for tips and troubleshooting.
This kit is BrdU-Red labeled (Ex/Em = 488/576nm). If want to use FITC (Ex/Em = 495/519nm) as a label, we recommend our In situ Direct DNA Fragmentation (TUNEL) Assay Kit (ab66108).
- Tested applicationsFluorescence Microscopy, Flow Cyt more details
- Storage instructionsPlease refer to protocols.
Components Identifier 60 tests 7-AAD/RNase Staining Buffer Amber bottle 1 x 30ml Anti-BrdU-Red Antibody Orange 1 x 300µl Br-dUTP Violet 1 x 480µl Negative Control Cells Neutral 1 x 5ml Positive Control Cells Brown 1 x 5ml Reaction Buffer Green 1 x 600µl Rinse Buffer Red 1 x 120ml TdT Enzymes 1 x 45µl Wash Buffer Blue 1 x 120ml
- Research Areas
- RelevanceInternucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.
Our Abpromise guarantee covers the use of ab66110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Fluorescence Microscopy||Fluorescence Microscopy|
|Flow Cyt||Flow Cyt|
References for In situ BrdU-Red DNA Fragmentation (TUNEL) Assay Kit (ab66110)
ab66110 has not yet been referenced specifically in any publications.