For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Abcam's In situ Direct DNA Fragmentation (TUNEL) Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The TUNEL-based detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-12-dUTP at the free 3'-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA can then be analyzed by flow cytometry.
Visit our FAQs page for tips and troubleshooting.
This kit is FITC-labeled (Ex/Em = 495/519nm). If want to use BrdU-Red (Ex/Em = 488/576nm) as a label, we recommend our In situ BrdU-Red DNA Fragmentation (TUNEL) Assay Kit (ab66110).
|FITC-dUTP||Orange||1 x 0.4ml|
|Negative Control Cells||Neutral||1 x 5ml|
|PI/RNase Staining Buffer||Amber bottle||1 x 25ml|
|Positive Control Cells||Brown||1 x 5ml|
|Reaction Buffer||Green||1 x 500µl|
|Rinse Buffer||Red||1 x 100ml|
|TdT Enzymes||1 x 38µl|
|Wash Buffer||Blue||1 x 100ml|
Our Abpromise guarantee covers the use of ab66108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|