Abcam's In situ Direct DNA Fragmentation (TUNEL) Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The TUNEL-based detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-12-dUTP at the free 3'-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA can then be analyzed by flow cytometry.
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This kit is FITC-labeled (Ex/Em = 495/519nm). If want to use BrdU-Red (Ex/Em = 488/576nm) as a label, we recommend our In situ BrdU-Red DNA Fragmentation (TUNEL) Assay Kit (ab66110).
|FITC-dUTP||Orange||1 x 0.4ml|
|Negative Control Cells||Neutral||1 x 5ml|
|PI/RNase Staining Buffer||Amber bottle||1 x 25ml|
|Positive Control Cells||Brown||1 x 5ml|
|Reaction Buffer||Green||1 x 500µl|
|Rinse Buffer||Red||1 x 100ml|
|TdT Enzymes||1 x 38µl|
|Wash Buffer||Blue||1 x 100ml|
Our Abpromise guarantee covers the use of ab66108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|