In situ Direct DNA Fragmentation (TUNEL) Assay Kit (ab66108)


  • Product nameIn situ Direct DNA Fragmentation (TUNEL) Assay Kit
    See all DNA fragments kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Product overview

    Abcam's In situ Direct DNA Fragmentation (TUNEL) Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The TUNEL-based detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-12-dUTP at the free 3'-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA can then be analyzed by flow cytometry.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This kit is FITC-labeled (Ex/Em = 495/519nm). If want to use BrdU-Red (Ex/Em = 488/576nm) as a label, we recommend our In situ BrdU-Red DNA Fragmentation (TUNEL) Assay Kit (ab66110).

  • Tested applicationsSuitable for: Flow Cytmore details


  • RelevanceInternucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.


Our Abpromise guarantee covers the use of ab66108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

In situ Direct DNA Fragmentation (TUNEL) Assay Kit images

  • Control RAW 264.7 cells.

  • RAW 264.7 cells treated with 2 μM camptothecin (ab120115) for 24 hours prior to staining.

  • RAW 264.7 cells treated with 10 μM camptothecin (ab120115) for 24 hours prior to staining.


References for In situ Direct DNA Fragmentation (TUNEL) Assay Kit (ab66108)

This product has been referenced in:
  • Chen J  et al. Antithrombin nanoparticles improve kidney reperfusion and protect kidney function after ischemia-reperfusion injury. Am J Physiol Renal Physiol 308:F765-73 (2015). Read more (PubMed: 25651565) »

See 1 Publication for this product

Product Wall

The laboratory responded to your inquiry regarding visibility of a pellet after adding the washing buffer. You should be able to see at least a mini-speck of the pellet even after the wash step. One easy thing to do would be to take an aliquot of ...

Read More

We have not tried this protocol on tissue sections, but here is a protocol that the lab has found from another which can be modified to fit into this procedure. A. Tissue Section Preparations: The protocol describes the preparation of formali...

Read More

Thank you for your inquiry and your patience while we looked into this further.

For ab66108, the kit components are optimized for usage on cells. If using tissue sections, you will not be able to get a full 50 assays from the kit because you ...

Read More

Thank you for contacting us. This kit will work fine with the samples you describe.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your email.

I discussed with my colleagues from the Scientific Support Team about the use of these kits in yeast and we agreed that the kit that is likely to work is the In situ Direct DNA Fragmentation Assay Kit (ab66108) but no...

Read More

Thank you for your enquiry and your interest in our products. The two kits (ab66108 and ab66110) are mainly used for cells analyzed by flow cytometry. Currently, we do not have specific kits for tissue samples (for DNA Fragmentation) but I would...

Read More