Potent, cell-permeable, selective calcineurin-NFAT interaction inhibitor (Kd = 800 nM). Blocks NFAT dephosphorylation, cytokine mRNA induction and nuclear import (~40 µM). Unlike CsA (ab120114) and FK506 (ab120223) INCA-6 does not indiscriminately block other calcineurin substrates. Anti-inflammatory agent. Active in vitro and in vivo.
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Soluble in DMSO to 25 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Frischbutter S et al. Evaluation of calcineurin/NFAT inhibitor selectivity in primary human Th cells using bar-coding and phospho-flow cytometry. Cytometry A81:1005-11 (2012).
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Sieber M & Baumgrass R Novel inhibitors of the calcineurin/NFATc hub - alternatives to CsA and FK506? Cell Commun Signal7:25 (2009).
Read more (PubMed: 19860902) »
Roehrl MH et al. Selective inhibition of calcineurin-NFAT signaling by blocking protein-protein interaction with small organic molecules. Proc Natl Acad Sci U S A101:7554-9 (2004).
Read more (PubMed: 15131267) »