• Product name
    Anti-INCENP antibody [58-217]
    See all INCENP primary antibodies
  • Description
    Mouse monoclonal [58-217] to INCENP
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    His-tagged human INCENP fragment aa231-298.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab23956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: 1/200. Perform heat mediated antigen retrieval using citrate buffer pH 6.0 before commencing with IHC staining protocol.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.
    • Sequence similarities
      Belongs to the INCENP family.
    • Cellular localization
      Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Nucleus. Chromosome > centromere > kinetochore. Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • binds and activates aurora B and C in vivo and in vitro antibody
      • Chromosomal passenger protein antibody
      • INCE_HUMAN antibody
      • INCENP antibody
      • Inner centromere protein antibody
      • Inner centromere protein antigens 135/155kDa antibody
      • Inner centromere protein antigens 135kD 155kD antibody
      • Inner centromere protein INCENP antibody
      see all


    • ICC/IF image of ab23956 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23956, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    • ab23956 staining INCENP in HeLa cells,  by immunofluorescence.

      optimal antibody dilution: 1mg/ml

      ab23956 staining INCENP in HeLa cells,  by immunofluorescence.

      optimal antibody dilution: 1mg/ml

      optimal fixation protocol: PFA/Triton fixation: 10 min room temperature in 3,7 % PFA diluted in PHEM PEM buffer: 45 mM Hepes pH 6,9, 45 mM Pipes pH 6,9, 5mM MgCl2, 10 mM EGTA) containing 0 ,2% Triton X-100, followed  by 3 washes in PBS.  Also gives a good staining with PFA fixation: 10 min in 3,7 % PFA  at room temperature, 5 min in PBS / 0,1%Triton X-100,  then 3 washes in PBS.

      IF staining was performed following a standard protocol: blocking: 30 min; primary antibody: 1 hr; secondary antibody: 45’. All incubations were at 37 °C in PBS/ 0,1% Tween containing 3% BSA.

      This antibody was also tested using NIH-3T3 cells but it did not provide good results with these cells.

    • ab23956 staining human anal cancer tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in citrate buffer (pH 6) prior to blocking in a commercially available blocking agent for 20 minutes at 20°C.  The primary anibody was diluted 1/200 and incubated with the sample for 40 minutes at 20°C.  A HRP-conjugated goat anti-mouse antibody was used as the secondary.

      See Abreview

    • ab23956 staining INCENP in siRNA-treated HeLa cells by Immunocytochemistry/ Immunofluorescence.


    This product has been referenced in:
    • Baron AP  et al. Probing the catalytic functions of Bub1 kinase using the small molecule inhibitors BAY-320 and BAY-524. Elife 5: (2016). Read more (PubMed: 26885717) »
    • Park H  et al. Ewing sarcoma EWS protein regulates midzone formation by recruiting Aurora B kinase to the midzone. Cell Cycle 13:2391-9 (2014). Read more (PubMed: 25483190) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (Colon carcinoma)
    Colon carcinoma
    Yes - 0.5% Triton in PBS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1%

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Dec 11 2012

    Abcam has not validated the combination of species/application used in this Abreview.
    Western blot
    Human Cell lysate - whole cell (HeLa, U2OS, Jurkat)
    Loading amount
    20 µg
    HeLa, U2OS, Jurkat
    Gel Running Conditions
    Reduced Denaturing (5% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Mr. Antibody Solutions

    Verified customer

    Submitted Aug 22 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (anal cancer)
    anal cancer
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
    Blocking step
    Sequential pre-diluted peroxidase (10 min) and protein (10 min) blocks. as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C

    Mr. Antibody Solutions

    Verified customer

    Submitted Jul 17 2008


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