Our Abpromise guarantee covers the use of ab12183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
Immunohistochemistry (Frozen sections) analysis of mouse embryonic skin - keratinocytes tissue sections labeling INCENP with ab12183. Tissue was fixed with paraformaldehyde, permeabilized with 10% Triton-X (0.05% Final) and blocked with Gelatin for 1 hour at 20°C. Samples were incubated with primary antibody (1/200) for 12 hours at 4°C. An undiluted Alexa Fluor 488 donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"