Overview

  • Product nameAnti-INCENP antibody
    See all INCENP primary antibodies
  • Description
    Rabbit polyclonal to INCENP
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human INCENP.

  • Positive control
    • This antibody gave a positive signal in Human Skin tissue lysate as well as the following whole cell lysates: A431; Caco2; MCF7.

Properties

Applications

Our Abpromise guarantee covers the use of ab86088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).

Target

  • FunctionComponent of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.
  • Sequence similaritiesBelongs to the INCENP family.
  • Cellular localizationChromosome > centromere. Cytoplasm > cytoskeleton > spindle. Nucleus. Chromosome > centromere > kinetochore. Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • binds and activates aurora B and C in vivo and in vitro antibody
    • Chromosomal passenger protein antibody
    • INCE_HUMAN antibody
    • INCENP antibody
    • Inner centromere protein antibody
    • Inner centromere protein antigens 135/155kDa antibody
    • Inner centromere protein antigens 135kD 155kD antibody
    • Inner centromere protein INCENP antibody
    see all

Anti-INCENP antibody images

  • All lanes : Anti-INCENP antibody (ab86088) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 106 kDa
    Observed band size : 106 kDa
    Additional bands at : 300 kDa,39 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-INCENP antibody (ab86088)

ab86088 has not yet been referenced specifically in any publications.

Product Wall

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