Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (ab110333)
Key features and details
- Mouse monoclonal [13G2AE2BH5] to PDHX
- Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5]
See all PDHX primary antibodies -
Description
Mouse monoclonal [13G2AE2BH5] to PDHX -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Isolated mitochondria from Human, Bovine, Rat and Mouse heart and HepG2 lysate; cultured, normal Human embryonic lung fibroblasts (strain MRC5); Human cerebellum tissue; HL60 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
ab110333 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
13G2AE2BH5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 , 69 kDa.
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ICC/IF | (2) |
Use a concentration of 1 µg/ml.
(heat-induced antigen-retrieval improves signal) |
Flow Cyt |
Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
IHC-P |
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 , 69 kDa. |
ICC/IF
Use a concentration of 1 µg/ml. (heat-induced antigen-retrieval improves signal) |
Flow Cyt
Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IHC-P
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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Function
Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. -
Involvement in disease
Defects in PDHX are the cause of pyruvate dehydrogenase E3-binding protein deficiency (PDHXD) [MIM:245349]. -
Sequence similarities
Belongs to the 2-oxoacid dehydrogenase family.
Contains 1 lipoyl-binding domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 8050 Human
- Entrez Gene: 27402 Mouse
- Entrez Gene: 311254 Rat
- Omim: 608769 Human
- SwissProt: O00330 Human
- SwissProt: Q8BKZ9 Mouse
- Unigene: 502315 Human
- Unigene: 315011 Mouse
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Alternative names
- Dihydrolipoamide dehydrogenase binding protein of pyruvate dehydrogenase complex antibody
- Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex antibody
- DLDBP antibody
see all
Images
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All lanes : Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (ab110333) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : DLAT knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HL-60 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 54 , 69 kDaLanes 1 - 4: Merged signal (red and green). Green - ab110333 observed at 72 kDa. Red - loading control, ab181602, observed at 38 kDa.
ab110333 was shown to specifically react with in wild-type HAP1 cells as signal was lost in DLAT knockout cells. Wild-type and DLAT knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab110333 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (ab110333) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Lane 5 : HepG2 cell lysate at 20 µg
Predicted band size: 54 , 69 kDa -
ab110333 staining Pyruvate dehydrogenase E2/E3bp in Human HUVEC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/1000 in PBS) for 1 hour. A CF568-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
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Immunocytochemistry analysis using ab110333 at 1µg/ml staining Pyruvate dehydrogenase E2/E3bp in cultured, normal Human embryonic lung fibroblasts and an AlexaFluor® 488 goat anti-mouse IgG2a secondary antibody (2 ug/ml).
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Immunohistological analysis using ab110333 at 1/1000 dilution staining Pyruvate dehydrogenase E2/E3bp in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
Note: Immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. -
Flow cytometric analysis using ab110333 at 1µg/ml staining Pyruvate dehydrogenase E2/E3b in HL60 cells (blue). Isotype control antibody (red).
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab110333 has been referenced in 41 publications.
- Ramirez A et al. Cannabidiol activates PINK1-Parkin-dependent mitophagy and mitochondrial-derived vesicles. Eur J Cell Biol 101:151185 (2022). PubMed: 34915361
- Yang Y et al. Altered succinylation of mitochondrial proteins, APP and tau in Alzheimer's disease. Nat Commun 13:159 (2022). PubMed: 35013160
- Wolf C et al. GDAP1 loss of function inhibits the mitochondrial pyruvate dehydrogenase complex by altering the actin cytoskeleton. Commun Biol 5:541 (2022). PubMed: 35662277
- Yagi M et al. Mitochondrial translation deficiency impairs NAD+ -mediated lysosomal acidification. EMBO J 40:e105268 (2021). PubMed: 33528041
- Lei Y et al. Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice. Sci Adv 7:N/A (2021). PubMed: 34039599