Recombinant Anti-Ube4a antibody [EPR7331] (ab128861)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7331] to Ube4a
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Ube4a antibody [EPR7331]
See all Ube4a primary antibodies -
Description
Rabbit monoclonal [EPR7331] to Ube4a -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, K562, Caco 2 and HepG2 cell lysates; Caco 2 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.91 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7331 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 123 kDa).
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ICC/IF | (1) |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 123 kDa). |
ICC/IF
1/250 - 1/500. |
Target
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Function
Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. -
Sequence similarities
Belongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain. - Information by UniProt
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Database links
- Entrez Gene: 9354 Human
- Entrez Gene: 140630 Mouse
- Entrez Gene: 315608 Rat
- Omim: 603753 Human
- SwissProt: Q14139 Human
- SwissProt: E9Q735 Mouse
- SwissProt: Q6P7A2 Rat
- Unigene: 602967 Human
see all -
Alternative names
- E4 antibody
- KIAA0126 antibody
- MGC133315 antibody
see all
Images
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All lanes : Anti-Ube4a antibody [EPR7331] (ab128861) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 123 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted? -
ab128861, at 1/100 dilution, staining Ube4a in Caco 2 cells by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab128861 has been referenced in 3 publications.
- Li C et al. NS5-V372A and NS5-H386Y variations are responsible for differences in interferon a/ß induction and co-contribute to the replication advantage of Japanese encephalitis virus genotype I over genotype III in ducklings. PLoS Pathog 16:e1008773 (2020). PubMed: 32881988
- Song C et al. AQR is a novel type 2 diabetes-associated gene that regulates signaling pathways critical for glucose metabolism. J Genet Genomics 45:111-120 (2018). WB ; Human . PubMed: 29502958
- Baranes-Bachar K et al. The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair. Mol Cell 69:866-878.e7 (2018). PubMed: 29499138