Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674)
Key features and details
- Mouse monoclonal [MCP231] to Proteasome 20S alpha 1+2+3+5+6+7
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] -
Description
Mouse monoclonal [MCP231] to Proteasome 20S alpha 1+2+3+5+6+7 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: a wide range of other species -
Immunogen
Full length protein corresponding to Proteasome 20S alpha 1+2+3+5+6+7. Dinitrophenylated proteasomes
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Epitope
Reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to alpha type subunits, although not necessarily identical in all. In general this motif is phylogenetically preserved. -
Positive control
- Human placental proteasome.
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General notes
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Dilute antibody to working dilution in PBS, pH 7.2 - 7.4. Store at 4°C and use within 1 month. Store unopened vial at -20°C for long term storage. Avoid freeze/thaw cycles.
This antibody reacts with six different alpha type subunits:- alpha 1, 2, 3, 5 , 6 and 7.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
This antibody reacts with six different alpha type subunits:- alpha 1, 2, 3, 5 , 6 and 7. -
Clonality
Monoclonal -
Clone number
MCP231 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
The proteasome is widely recognised as the central enzyme of non lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP dependent pathway of protein degradation. The catalytic core of this unusually large complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, alpha and beta, each group having distinct structural and functional roles. The alpha subunits comprise the outer rings and the beta subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (alpha7/beta7/beta7/alpha7) with a member of each sub family represented in each particle. Each subunit is located in a unique position within the alpha or beta rings. 20S Proteasomes degrade only unfolded proteins in an energy independent manner, whereas 26S proteasomes degrade native and ubiquitinylated proteins in an ATP dependent manner. The native protein substrates are recognised by subunits, some with ATP binding sites, of the outer 19S caps of the 26S proteasome. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 5682 Human
- Entrez Gene: 5683 Human
- Entrez Gene: 5684 Human
- Entrez Gene: 5686 Human
- Entrez Gene: 5687 Human
- Entrez Gene: 5688 Human
- Omim: 176842 Human
- Omim: 602854 Human
see all -
Alternative names
- Proteasome component antibody
- Proteasome subunit alpha type 1 antibody
- Proteasome subunit alpha type 2 antibody
see all
Images
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ICC/IF image of ab22674 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22674, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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Overlay histogram showing HepG2 cells stained with ab22674 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22674, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab22674 has been referenced in 24 publications.
- Kammerl IE et al. Activation of immune cell proteasomes in peripheral blood of smokers and COPD patients: implications for therapy. Eur Respir J 59:N/A (2022). PubMed: 34561290
- Wong DCS et al. CRYPTOCHROMES promote daily protein homeostasis. EMBO J 41:e108883 (2022). PubMed: 34842284
- Bonhoure A et al. Extracellular 20S proteasome secreted via microvesicles can degrade poorly folded proteins and inhibit Galectin-3 agglutination activity. Traffic 23:287-304 (2022). PubMed: 35466519
- Schlesser C et al. Metformin Induces Resistance of Cancer Cells to the Proteasome Inhibitor Bortezomib. Biomolecules 12:N/A (2022). PubMed: 35740881
- Biagioni F et al. The Autophagy-Related Organelle Autophagoproteasome Is Suppressed within Ischemic Penumbra. Int J Mol Sci 22:N/A (2021). PubMed: 34638703