Anti-PDI1 antibody [38H8] (ab4644)
Key features and details
- Mouse monoclonal [38H8] to PDI1
- Suitable for: ICC/IF, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-PDI1 antibody [38H8] -
Description
Mouse monoclonal [38H8] to PDI1 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Tissue, cells or virus corresponding to PDI1. Yeast nuclear preparations
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
>90% pure based on SDS-PAGE analysis. -
Clonality
Monoclonal -
Clone number
38H8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/500 - 1/5000.
(gives an ER staining pattern on yeast cells). |
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 58.2 kDa).
Suggested working dilution of 1/5000 (see PMID: 17051412). |
Notes |
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ICC/IF
1/500 - 1/5000. (gives an ER staining pattern on yeast cells). |
WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 58.2 kDa). Suggested working dilution of 1/5000 (see PMID: 17051412). |
Target
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Relevance
PDI1 is a protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds. -
Database links
- SwissProt: P17967 Saccharomyces cerevisiae
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Alternative names
- MFP1 antibody
- PDI1 antibody
- Pdi1p antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab4644 has been referenced in 5 publications.
- Pal A et al. Eisosome protein Pil1 regulates mitochondrial morphology, mitophagy, and cell death in Saccharomyces cerevisiae. J Biol Chem 298:102533 (2022). PubMed: 36162502
- Vakirlis N et al. De novo emergence of adaptive membrane proteins from thymine-rich genomic sequences. Nat Commun 11:781 (2020). PubMed: 32034123
- Yi T et al. LncRNA LL22NC03-N14H11.1 promoted hepatocellular carcinoma progression through activating MAPK pathway to induce mitochondrial fission. Cell Death Dis 11:832 (2020). PubMed: 33028809
- Delic M et al. Oxidative protein folding and unfolded protein response elicit differing redox regulation in endoplasmic reticulum and cytosol of yeast. Free Radic Biol Med 52:2000-12 (2012). WB . PubMed: 22406321
- Damasceno LM et al. Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris. Appl Microbiol Biotechnol 74:381-9 (2007). PubMed: 17051412