Recombinant Hepatitis B Virus Core Antigen protein (ab49014)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: WB, ELISA
Description
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Product name
Recombinant Hepatitis B Virus Core Antigen protein
See all Hepatitis B Virus Core Antigen proteins and peptides -
Biological activity
Reacts strongly with human HBV positive serum. -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino acids
1 to 144 -
Additional sequence information
Deleted DNA binding domain. Fully competent for self-assembly.
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Specifications
Our Abpromise guarantee covers the use of ab49014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
ELISA
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Form
Liquid -
Additional notes
During infection, the C gene of HBV often displays genetic deletions within the tip of the protruding spikes of HBcAg, which are known to contain the major site for antibody binding . These have been referred to as core internal deletion variants, and they often appear in end stage liver disease. Depending on the nature of the deletion, they may still form functional capsids,
Reacts strongly with human HBV positive serum. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.20
Constituents: 0.02% PBS, 50% Glycerol (glycerin, glycerine), 0.435% Sodium chloride
General Info
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Alternative names
- C
- Capsid protein
- core
see all -
Relevance
Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection. -
Cellular localization
Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab49014 has been referenced in 2 publications.
- Colant N et al. Escherichia Coli-Based Cell-Free Protein Synthesis for Iterative Design of Tandem-Core Virus-Like Particles. Vaccines (Basel) 9:N/A (2021). PubMed: 33669126
- Granovski V et al. Humoral Immune Response in Mice Elicited by Combined Yeast-Derived Hepatitis B Virus Core, Surface, and Mosaic Surface Antigens. Intervirology 60:90-101 (2017). PubMed: 28957812