Fast Red Substrate Kit (ab64254)
Overview
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Product name
Fast Red Substrate Kit -
Product overview
Fast Red Substrate kit ab64254 for immunohistochemical staining using alkaline phosphatase-based detection.
Fast Red can be used as chromogen for immunohistochemical staining. In the presence of alkaline phosphatase enzyme, Fast Red produces a red reaction product that can be seen using either brightfield or fluorescent microscopy.
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Notes
This product was previously called Liquid Fast Red Substrate Kit.
Streptavidin - Alkaline phospatase ab246833 is suitable for use with Fast red substrate.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Tested applications
Suitable for: IHC-P, WBmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 125 ml 60 ml 75x Fast-Red Chromogen 1 x 2ml 1 x 1ml Liquid Fast Red Substrate 1 x 125ml 1 x 60ml -
Research areas
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Relevance
Fast-Red is a widely used chromogen which produces a red reaction product in the presence of alkaline phosphatase enzyme.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent dilution.
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WB |
Use at an assay dependent dilution.
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Notes |
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IHC-P
Use at an assay dependent dilution. |
WB
Use at an assay dependent dilution. |
Images
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab64254 has been referenced in 13 publications.
- Zakeri N et al. Characterisation and induction of tissue-resident gamma delta T-cells to target hepatocellular carcinoma. Nat Commun 13:1372 (2022). PubMed: 35296658
- Murphy BM et al. Enhanced BRAF engagement by NRAS mutants capable of promoting melanoma initiation. Nat Commun 13:3153 (2022). PubMed: 35672316
- van Wijk LM et al. The RAD51-FFPE Test; Calibration of a Functional Homologous Recombination Deficiency Test on Diagnostic Endometrial and Ovarian Tumor Blocks. Cancers (Basel) 13:N/A (2021). PubMed: 34203855
- Paijens ST et al. Prognostic image-based quantification of CD8CD103 T cell subsets in high-grade serous ovarian cancer patients. Oncoimmunology 10:1935104 (2021). PubMed: 34123576
- Acharya M et al. Production and characterization of avian crypt-villus enteroids and the effect of chemicals. BMC Vet Res 16:179 (2020). PubMed: 32503669