Caspase 9 Assay Kit (Colorimetric) (ab65608)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
Overview
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Product name
Caspase 9 Assay Kit (Colorimetric)
See all Caspase-9 kits -
Detection method
Colorimetric -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Enzyme activity -
Assay time
2h 00m -
Species reactivity
Reacts with: Mammals -
Product overview
Caspase 9 Assay Kit (Colorimetric) ab65608 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD.
The caspase 9 assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate LEHD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K119 Caspase-9 Colorimetric Assay Kit. K119-100 is the same size as the 100 test size of ab65608.
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2X Reaction Buffer I 4 x 2ml Dilution Buffer II 1 x 100ml DTT I 1 x 400µl LEHD-pNA 1 x 500µl Lysis Buffer IV 1 x 100ml -
Research areas
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Relevance
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex. -
Alternative names
- APAF3
- CASP9
- Caspase 9
see all
Associated products
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Related Products
Images
Datasheets and documents
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SDS download
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Datasheet download
References (64)
ab65608 has been referenced in 64 publications.
- Gan K et al. Positive feedback loop of lncRNA FAM201A/miR-146a-5p/POU2F1 regulates IL-1ß-induced chondrocyte injury in vitro. Mol Med Rep 25:N/A (2022). PubMed: 34796909
- Kirman DC et al. Cell surface nucleolin is a novel ADAMTS5 receptor mediating endothelial cell apoptosis. Cell Death Dis 13:172 (2022). PubMed: 35197459
- Lai MC et al. The Citrus Flavonoid Hesperetin Encounters Diabetes-Mediated Alzheimer-Type Neuropathologic Changes through Relieving Advanced Glycation End-Products Inducing Endoplasmic Reticulum Stress. Nutrients 14:N/A (2022). PubMed: 35215394
- Bashir R et al. The modulation of PI3K/Akt pathway by 3β hydroxylup-12-en-28-oic acid isolated from Thymus linearis induces cell death in HCT-116 cells. Chem Biol Drug Des 99:162-178 (2022). PubMed: 34558199
- Mohamed ME et al. The Protective Effect of Anethole against Renal Ischemia/Reperfusion: The Role of the TLR2,4/MYD88/NF?B Pathway. Antioxidants (Basel) 11:N/A (2022). PubMed: 35326185