Our Scientific Support and Customer Services teams are on hand to reply to your questions

1. Inquiry type -- Please choose your inquiry type -- Looking for a product Problem with a product Request a bulk quote Website inquiry App inquiry Careers inquiry Licensing inquiry Question about an order
2. Application -- Select an application -- ChIP ELISA Flow Cytometry Kit IHC IP WB Other
4. First name
5. Last name
6. Email
7. Phone (optional)
8. Product code (optional)
9. Lot number (optional)
10. Please describe the problem experienced and tell us as much information as possible about your experiment (description of problem, sample preparation and species, experimental conditions, controls used, any troubleshooting you have attempted, whether this product has been used successfully in the past), including:
11. Inquiry
    The more information you provide, the easier it will be for us to assist with your inquiry…
    
    Product type, species, quantity etc.

    Please tell us as much information as possible about your experiment ; the conditions, the controls, any troubleshooting tips that you have attempted …. E.g.

    • Sample preparation
    • Electrophoresis and transfer conditions
    • Blocking conditions
    • Primary antibody conditions
    • Secondary antibody conditions
    • Wash steps
    • Detection

    Interested in larger quantities? Contact us for a quote.

    Need help with the website or noticed something you'd like to comment on? Just let us know.

    Need help with the mobile app or noticed something you'd like to comment on? Just let us know.

    Interested in any of our vacancies? Just let us know which position and we'll get back to you.

    If you have excellent quality products, we'd be delighted to market and distribute these for you.

    Like to ask us about something else?

    ChIP
    

    • DNA detection method (regions amplified in PCR, microarray, direct sequencing)
    • DNA purification (decrosslinking conditions, DNA purification method)
    • Immunoprecipitation conditions (buffer, amount of detergent, primary antibody manufacturer/dilution, incubation time, matrix for isolating antibody complex)
    • Sample preparation (X-ChIP/N-ChIP, cross-linking conditions, DNA fragment size)
    • Wash steps

    ELISA
    

    • Amount of protein used
    • Blocking conditions
    • Detection method
    • Primary antibody conditions (diluent/dilution, incubation time/temperature)
    • Secondary antibody conditions (diluent/dilution, incubation time/temperature)
    • Type of ELISA (e.g. direct, indirect, sandwich ELISA)
    • Wash steps
    • What did the standard curve look like?

    Flow Cytometry
    

    • Blocking/fixation conditions
    • Detection and analysis method
    • Number of cells used
    • Primary antibody conditions (diluent/dilution, incubation time/temperature)
    • Secondary antibody conditions (diluent/dilution, incubation time/temperature)
    • Wash steps

    Kit
    

    • Primary antibody conjugation conditions (amount, isotype, buffer, incubation time/temperature)
    • Sample preparation, treatment(s) (sample type, method of protein removal)
    • Storage conditions of kit components
    • Was the exact protocol followed (as supplied with the kit)?
    • Which kit component is causing the issue (enzyme/developer/standard)

    IHC
    

    • Blocking conditions
    • Detection method, amplification steps (if any)
    • Primary antibody conditions (diluent/dilution, incubation time/temperature)
    • Sample fixation, antigen retrieval, permeabilization
    • Secondary antibody conditions (diluent/dilution, incubation time/temperature)
    • Wash steps

    IP
    

    • Amount of protein used
    • Detection method
    • Matrix used for pull-down
    • Primary antibody conditions (diluent/dilution, incubation time/temperature)
    • Wash steps
    • Western blot conditions

    WB
    

    • Amount of protein loaded
    • Blocking conditions
    • Detection method
    • Electrophoresis and transfer conditions
    • Primary antibody conditions (diluent/dilution, incubation time/temperature)
    • Secondary antibody conditions (diluent/dilution, incubation time/temperature)
    • Wash steps

    Other
    

    • Blocking conditions, if applicable
    • Description of the experiment you are running, with assay conditions (protocol summary, concentrations, incubation conditions)
    • Detection method
    • Primary antibody conditions (diluent/dilution, incubation time/temperature)
    • Sample preparation (amount of protein /cell number /fixation or permeabilization conditions)
    • Secondary antibody conditions (diluent/dilution, incubation time/temperature)
12. Upload file (optional)