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The following primers were used to test ab6002 by chromatin immunoprecipitation (ChIP)
Quantitative PCR is performed using the Taqman approach to amplify the loci shown. Primers are shown in capital letters and the probes in lower case. Primers were all used at 0.9uM final and the probes at 0.25uM final.
BC025925 (accession number)
hsGAPDH exon 1 F1 TCG ACA GTC AGC CGC ATC T
hsGAPDH intron 1 R1 CTA GCC TCC CGG GTT TCT CT
hsGAPDH exon 1 tcg cca ggt gaa gac ggg cg
BC0327000 (accession number)
hsRPL30 first exon F1 CAA GGC AAA GCG AAA TTG GT
hsRPL30 first exon R1 GCC CGT TCA GTC TCT TCG ATT
hsRPL30 exon 1 tct cgc taa caa ctg ccc agc ttt gag
BC004333 (accession number)
hsALDOA second intron F TCC TGG CAA GAT AAG GAG TTG AC
hsALDOA second intron R1 ACA CAC GAT AGC CCT AGC AGT TC
hsALDOA intron 2 atc cac gag aaa agg cat ttc cgg c
X56677 (accession number)
hsMYO-D exon 1 F1 CCG CCT GAG CAA AGT AAA TGA
hsMYO-D exon 1 R1 GGC AAC CGC TGG TTT GG
hsMYO-D exon 1 tca agc gct gca cgt cga gca at
AF113676 (accession number) - Liver specific
hsSERPINA1 (anti trypsin) intron 1 F1 GGC TCA AGC TGG CAT TCC T
hsSERPINA1 (anti trypsin) intron 1 R1 GGC TTA ATC ACG CAC TGA GCT TA
hsSERPINA1 (anti trypsin) intron 1 ggc tca agc tgg cat tcc t
X72623 (accession number) - Heterochromatin
hsSat2 repeat F1 ATC GAA TGG AAA TGA AAG GAG TCA
hsSat2 repeat F1 GAC CAT TGG ATG ATT GCA GTC A
M26919 (accession number) - Heterochromatin
hsSat alpha F1 AAG GTC AAT GGC AGA AAA GAA
hsSat alpha R1 CAA CGA AGG CCA CAA GAT GTC
The PCR is performed with the TaqMan Universal PCR Master Mix from ABI.
Amplification:
Stage 1 - 2 min at 50oC 1 repetition
Stage 2 - 10 mins at 95oC 1 repetition
Stage 3 - 30 sec at 95oC, 1 min at 60oC 35 repetitions
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