For the first time, whole cell lysates from embryonic stem cell lines are available!
Read on to find out more about these exciting new additions to our Stem Cells range...
|There are currently no regulations preventing the use of NIH funding for purchase of lysates/extracts from "non-approved" embryonic stem cell lines for research purposes.|
MEL-1 and MEL-2 are Australian normal human male (46XY) and female (46XX) embryonic stem cell lines respectively. Each cell line was grown on gamma-irradiated mouse foreskin fibroblast feeder cells and less than 10% of the cells from which the lysate was derived were feeder cells. The production of MEL-1 and MEL-2 was ethically approved and they have now been accepted for deposition in the UK stem cell bank.
WB - Whole Cell Stem Cell Lysates
All lanes : Oct4 antibody (ab19857) at 1 ug/ml
Lysates/proteins at 10 ul per lane.
The 46C and IOUD2 (below) lysates were both produced in the lab of Austin Smith. This lysate was taken from Sox1-positive neural precursor cells derived from mouse embryonic stem cells. The Sox1 selection technology is patented. Sox1-positive cells were selected using puromycin following growth of mouse ES cells in N2B27 neural differentiation media. The cell line was first described in:
Jirmanova L et al. Differential contributions of ERK and PI3-kinase to the regulation of cyclin D1 expression and to the control of the G1/S transition in mouse embryonic stem cells. Oncogene 2002 Aug 15;21(36):5515-28