ICC/IF - SSEA 4 antibody [MC813] |
NTERA2 Human Embryonic Carcinoma cells were stained with SSEA4 antibody (ab16287). As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst. |
The Stem Cells team have been working hard to produce the hottest new antibodies to add to our growing range of Stem Cell markers. Here are just a few of our latest additions.
Watch this space for other new additions coming soon!
SSEA4 antibody [MC813] (ab16287)
Mouse monoclonal
Immunogen: Human embryonal carcinoma cell line 2102Ep.
Reacts with: Human and mouse
Tested in: Immunohistochemistry, Flow Cytometry / FACS, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence
Specificity: This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4, a glycolipid carbohydrate epitope) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
TRA-1-60 antibody [TRA-1-60] (ab16288)
Mouse monoclonal
Immunogen: Human embryonal carcinoma cell line 2102Ep cl.2A6.
Reacts with: Human
Tested in: Western blot, Immunoprecipitation, Radioimmunoassay, Immunohistochemistry (Frozen sections), Immunofluorescence, Immunocytochemistry, Flow Cytometry / FACS
Specificity: This antibody reacts with the TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule. The epitopes for both TRA-1-81 and TRA-1-60 are not confined to human EC cells, but are also found on several neoplastic and normal tissues, though in a different pattern for each antibody. TRA-1-81 immunoprecipitates a 420kDa and a 250kDa protein and TRA-1-60 immunoprecipitates a 235kDa and a 410kDa protein from lysates of human EC cells.
View TRA-1-60 antibody datasheet
Brachyury / Bry antibody (ab20680) - Mesoderm Marker
Rabbit polyclonal
Immunogen: Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Brachyury.
Reacts with: Human. Predicted to react with Mouse (81% identity with immunogen) and Dog (87% identity with immunogen) due to sequence homology.
Tested in: Immunocytochemistry/Immunofluorescence
Relevance: This transcription factor is the earliest indicator of mesoderm formation. Used as a marker of mesodermal differentiation, Brachyury is expressed during gastrula and neurula stages in involuting mesoderm and in the notochord.
View Brachyury antibody datasheet
Immunocytochemistry/Immunofluorescence - Brachyury / Bry antibody - Mesoderm Marker (ab20680) Ludovic Valllier, University of Cambridge, UK
Musashi 1 / Msi antibody (ab21628)
Rabbit polyclonal
Immunogen: Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Musashi1 / Msi1.
Reacts with: Mouse. Predicted to react with Human (100% identity with immunogen), Rat (100% identity with immunogen) and Xenopus laevis (92% identity with immunogen) due to sequence homology.
Tested in: Western blot
Relevance: In mammals the Musashi family is important for cell fate determination, playing roles in maintenance of the stem cell state, differentiation and tumorigenesis. Musashi1 (also known as Msi1), is selectively expressed in neural progenitor cells, including neural stem cells (Sakakibara et al., 1996; Kaneko et al., 2000). Outside the nervous system, Musashi1 is a selective marker for intestinal stem or early lineage cells. Musashi1 interacts with the Notch pathway during asymmetric cell division by binding the 3' UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi1 is present.
View Musashi 1 antibody datasheet
REST antibody (ab21635)
Rabbit polyclonal
Immunogen: Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Human REST.
Reacts with: Human. Predicted to react with Mouse (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology.
Tested in: Western blot
Relevance: The proposed role of REST is that of a transcriptional silencer that restricts neuronal gene expression to the nervous system by silencing expression of these genes in non-neural tissues (Bruce et al., 2004).
Rabbit polyclonal
Immunogen: Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Islet 1.
Reacts with: Human, Mouse and Rat. Predicted to react with Zebrafish (100% identity with immunogen) and Chicken (100% identity with immunogen) due to sequence homology.
Tested in: Immunohistochemistry (Frozen sections), Immunocytochemistry/Immunofluorescence
Relevance: Islet 1 is encoded by ISL1 and is a marker od Neural Stem Cells. Islet 1 is a transcription factor containing two amino-terminal LIM domains and one carboxy-terminal homeodomain. Islet 1 and ISL1 play an important role in the embryogenesis of pancreatic islets of Langerhans. In addition, mouse embryos made deficient in ISL1 fail to undergo neural tube motor neuron differentiation.