At Abcam we now have a panel of antibodies against the major epitope tags; HA, Myc, His, T7 and V5 for use in chromatin immunoprecipitation (ChIP).
ChIP Grade Tag Antibodies
AbID | Target | Epitope Tag |
9110 | Rabbit polyclonal to HA Tag | HA |
1424 | Mouse monoclonal [4C12] to HA tag | HA |
9132 | Goat polyclonal to Myc tag | Myc |
9108 | Rabbit polyclonal to 6xHis tag | His |
9138 | Goat polyclonal to T7 tag | T7 |
15828 | Rabbit polyclonal to V5 tag | V5 |
We have validated our antibodies against the epitope tags using a human cell line stably transfected with the Gal4 upstream activation sequence. Cells were transiently transfected with epitope tagged Gal4 DNA Binding Domain constructs. Our epitope tagged antibodies were assayed for their capacity to chromatin immunoprecipitate by performing ChIP in parallel using antibodies against Gal4 (ChIP grade; ab1396) and the respective epitope tag. The enrichment across the Gal4 upstream activation sequence was assayed by real time PCR.
The results (see figure below for example) showed that the antibodies that were confirmed as working in ChIP demonstrated a similar level of enrichment at the Gal4 upstream activation sequence using anti-Gal4 antibody (ab1396) to the epitope tagged (anti-T7 and anti-V5) antibodies.
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Abcam now has a comprehensive panel of ChIP grade antibodies against the major epitope tags for use in a broad range of experimental systems. At Abcam we make a promise (our Abpromise) to our customers that our antibodies behave as detailed on our datasheets - and, where ChIP-Grade is noted, that means we guarantee that all of our new epitope tagged antibodies work by chromatin immunoprecipitation too!
